GFP and GAL

Martin Poot MARTIN at probes.mhs.compuserve.com
Fri Jan 26 19:51:32 EST 1996


Dear Flow-ers,

The question is whether there is a red fluorogenic substrate for
beta-galactosidase activity monitoring.  Yes, there is, but there seems to
be a lot of trouble with the resorufin based material.  If you have access
to UV excitation I would rather recommend a chloromethylcoumarin-based
substrate.  This one is available from Molecular Probes, Inc. (Tel: (541)
465-8300 / FAX: (541) 344-6504) under catalog number D-2921.  Disclaimer: 
I have not tried it myself.

Good luck !

Martin Poot

martin at probes.mhs.compuserve.com




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