Membrane polarization

Jan Bly bly at umsmed.edu
Mon Apr 15 23:10:01 EST 1996


	We are trying to measure membrane polarization by flow cytometry 
using the probe TMA-DPH.  So far we've had no luck.  Is anyone else doing 
this?  If so, can you tell me which probes you have found most successful 
and the specs on your polarization filters.  We are using a FACS Vantage 
and "hand-me-down" polarizers (no specs).  The polarizers we're using are 
yellow, and we're wondering if we were just cutting out most of the 
TMA-DPH emitted signal.
	TIA for any help,   Jan Bly



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