flourescent signal shifts

Jason, Janine jmj1 at CIDHIV7.EM.CDC.GOV
Fri Jun 9 15:46:00 EST 1995

To people doing intracellular (directly conjugated) and surface 
staining(also directly conjugated):

Do you find you have flourescent signal shifts doing the above, vs doing all 
surface staining (3-color)?  If so, do you have any idea why it happens?  If 
not, could you share with me your protocol for intracellular staining?

Many thanks,

Janine Jason

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