BU-1 clone

KLIN@stem.com KLIN at stem.com
Wed Jul 26 13:19:23 EST 1995

I tried a protocol combing surface staining and BRDU of activated lymphocytes. 
The method used hypotonic solution to permeablize cells instead of alcohol.  
Since it is a very mild permeablization, the surface marker could be preserved.  
I got very good surface staining.  My problem was that I could not get BRDU 
stain.(Actually, I got once.). I tried different time or concentrations of BRDU 
pulsing and new vial of BU-1 antibody from Amersham but no sucess. I am still 
interested to find out why.  Your explanation may ansewer the question.Thanks.


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