EUROPIUM CHELATES

dbk dbk at aber.ac.uk
Fri Nov 11 11:46:13 EST 1994


     In message <9411102245.AA17698 at flowcyt.cyto.purdue.edu>,PAUL at flowcyt.cyto.
    purdue.edu writes:
     *Graeme Vessey asks:
     *
     *> DOES ANYONE KNOW OF A SUPPLIER FOR EUROPIUM CHELATES SUITABLE FOR 
     *> CONJUGATING TO ANTIBODIES.  WE ARE INTERESTED IN USING THESE REAGENTS F
     *OR 
     *> TIME RESOLVED FLUORESCENCE IN FLOW FOR DETECTING VERY SMALL PARTICLES. 
     * 
     *> 
     *> HAS ANYONE GOT EXPERIENCE WITH TIME RESOLVED FLUORESCENCE IN FLOW.  
     *> 
     *> ANY SUGESTIONS FOR FLUOROCHROMES WITH LONG FLUORESCENCE LIFETIMES.
     *> REGARDS,
     *Guenter Valet has published data on flow cytometry using europium 
     *chelates at least in abstract form I can't remember if it was 
     *published elsewhere - also Seveus published a paper in Cytometry 
     *13:329 (1992) on imaging and time resolved fluorescence.
     *
     *Paul Robinson
     *
     *J.Paul Robinson, Purdue University Cytometry Labs
     *
     *
and 2 papers in Cytometry 16, 187-94 and 195-205 (1994) by Condrau et al
at Zuerich describe making a flow cytometer for this. The abstract of the
second paper describes using a Europium chelate, and presumably where
they got it (I don't have the original to hand).

Anyone any other views on whether this is the way to go to get more
parameters per laser, and how to modify a commercial machine to do it?

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*Douglas B. Kell                            Phone: +44 1970 622334 *
*Institute of Biological Sciences           Fax:   +44 1970 622354 *
*University of Wales,                        Email: DBK at ABER.AC.UK *
*Aberystwyth SY23 3DA, U.K.         http://144.124.112.51/home.htm *
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Douglas.




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