Dr Stephen Young s.p.young at
Mon Jan 31 02:55:38 EST 1994

Kris I was interested to see that there was a solution to your problem with
the big files.

> There is one 11 MByte listmode data file on there that is a calcium flux
> plus surface marker.  (We were interested in the PE+ only.)  I don't
> remember whether or not this one fluxed, but at least you'll be able to
> see if you can manipulate the file.

We are also measuring calcium fluxes but have kept away from attempting
simultaneous surface labelling becuase of the possible effects of the
antibody on the subsequent calcium flux. How do you get over this? and which
surface proteins are you labelling?

I'd be interested to hear.


Steve Young
Dr Stephen Young, Dept of Rheumatology, University of Birmingham
Email:  s.p.young at
Phone:  021-414-6480
FAX:    021-414-6794

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