Replies to following inquiry

USA::SW11527 SW11527%USA.decnet at usav01.glaxo.com
Sat Sep 25 17:41:00 EST 1993


>From: sanjeev at nii.ernet.in

>To: cytometry at flowcyt.cyto.purdue.edu
>Subject: A FACS related problem
>
>A certain molecule is expressed on the surface of two different cell lines at 
>similar levels, but (probably) in two different conformations. I have a 
>monoclonal that (probably!) discriminates between the two conformations. In 
>comparing the staining pattern of these two cell lines  I face the following 
>problem.
>The (no first Ab) conjugate controls for these two cell lines stain at two 
>different levels, so that it becomes impossible to compare the test 
>stainings. 
>
>1)If I change the voltage for one cell line to  match its control staining to 
>that of the other, won't it bring about a more dramatic (and unreal) effect 
>on the test staining for this particular cell line?
>2)Or can I just superimpose the two control stainings and compare then compare 
>the stainings with the monoclonal in question?
>If anyone could throw some light on this aspect of FACS data analysis I will 
>be really greatful.
>Pl. send your replies at the following address:

>$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$
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>        SANJEEV KUMAR                             TEL>91-11-6863004
>        IMMUNOBIOLOGY LABS                        FAX>91-11-6862125
>        NATIONAL INSTITUTE OF IMMUNOLOGY        TELEX>031-66183 NII IN
>        ARUNA ASAF ALI MARG                     GRAMS>IMMUNOLOGY
>        NEW DELHI-67                            EMAIL> Sanjeev at NII.ernet.in
>
>$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$  
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>
	
	I for one would also like to see some of the suggestions.

Perhaps they could be CC'd to the mailing list?

				Cheers,

				Sam Witherspoon
				Pharmacology
				Glaxo Inc
				
				sw11527 at usav01.glaxo.com






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