Replies to following inquiry

USA::SW11527 SW11527%USA.decnet at
Sat Sep 25 17:41:00 EST 1993

>From: sanjeev at

>To: cytometry at
>Subject: A FACS related problem
>A certain molecule is expressed on the surface of two different cell lines at 
>similar levels, but (probably) in two different conformations. I have a 
>monoclonal that (probably!) discriminates between the two conformations. In 
>comparing the staining pattern of these two cell lines  I face the following 
>The (no first Ab) conjugate controls for these two cell lines stain at two 
>different levels, so that it becomes impossible to compare the test 
>1)If I change the voltage for one cell line to  match its control staining to 
>that of the other, won't it bring about a more dramatic (and unreal) effect 
>on the test staining for this particular cell line?
>2)Or can I just superimpose the two control stainings and compare then compare 
>the stainings with the monoclonal in question?
>If anyone could throw some light on this aspect of FACS data analysis I will 
>be really greatful.
>Pl. send your replies at the following address:

>                               /-----------\
>                               |    \\//   |
>                               |     ||    |
>                               |  \\//\\// |
>                               |   ||  ||  |
>                               \-----------/
>        SANJEEV KUMAR                             TEL>91-11-6863004
>        IMMUNOBIOLOGY LABS                        FAX>91-11-6862125
>        ARUNA ASAF ALI MARG                     GRAMS>IMMUNOLOGY
>        NEW DELHI-67                            EMAIL> Sanjeev at
	I for one would also like to see some of the suggestions.

Perhaps they could be CC'd to the mailing list?


				Sam Witherspoon
				Glaxo Inc
				sw11527 at

More information about the Cytometry mailing list